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Read more about this product. The antibody–Fab conjugate was added to the serum–biotin-Avidin mixture, and the mixtures were incubated for 30 min at 37°C. A biotinylated HRP-labeled anti–mouse IgM (T15-478-2; Jackson ImmunoResearch Laboratories) was added and incubated for 30 min at 37°C. Biotinylated HRP was detected with TMB substrate (Sigma-Aldrich). eea19f52d2

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Statistical analysis.

P-values were calculated with the Student’s *t* test or the Mann-Whitney test, with one or two-tailed *P* values, respectively. Error bars represent the SEM.

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